Journal: PLOS One

Article Title: Impact of blast exposure on visual pathway: Mechanism exploration and novel diagnostic perspectives

doi: 10.1371/journal.pone.0344993

Figure Lengend Snippet: (A) Schematic diagram of the visual pathway and key observation areas in its intracranial segment. (B) Representative images of immunofluorescence staining for NeuN (green) and NLRP3 (red), Iba-1 (grey) in mouse visual cortex slices, Group information is shown in images. The red triangle marks indicate the co-localization of NLRP3 and NeuN. The scale bars for all fluorescence intensity channels and the merge are set at 50 μm, the magnified inset is 20μm. Quantitative analysis of visual cortex immunofluorescence density in Iba-1 (C) and NLRP3 (G) . (H) Fluorescence intensity of NeuN + NLRP3 + / NeuN + , n = 4. (D, E, F, I, J) Western blot analysis of Iba-1, IL-1β, cleaved Gasdermin D and NLRP3 in visual cortex lysates, the molecular mass is indicated in kilodaltons, n = 3. (K-L) Quantitative analysis of representative fluorescence images of NeuN staining with TUNEL labeling in the peri-contusion region from different groups that information is shown in images, Scale bar = 100 μm, n = 4. (M) Quantitative analysis of visual cortex NeuN + cells, n = 4. Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05.

Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with gentle agitation using the following primary antibodies: anti-MBP (1:10,000; CST) anti-Iba-1 (1:10,000; CST), anti-NLRP3 (1:10,000; Invitrogen), anti-IL-1β (1:8,000; CST, # 12242) anti-cleaved Gasdermin D (1:10,000, CST, # 10173), and anti-GAPDH (1:10,000, Abcam, # ab9485).

Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot, TUNEL Assay, Labeling

Journal: PLOS One

Article Title: Impact of blast exposure on visual pathway: Mechanism exploration and novel diagnostic perspectives

doi: 10.1371/journal.pone.0344993

Figure Lengend Snippet: (A) Representative images of immunofluorescence staining for NeuN (green), NLRP3 (red), and Iba-1 (grey) in mouse visual cortex slices, as well as group information, are shown in the images. The red triangle marks indicate the co-localization of NLRP3 and NeuN. The scale bars for all fluorescence intensity channels and the merge are set at 50 μm, the magnified inset is 20 μm, n = 4. (B) Quantitative analysis of visual cortex immunofluorescence density in NLRP3, n = 4. (C) Fluorescence intensity of NeuN + NLRP3 + /NeuN + , n = 4. (D) Quantitative analysis of visual cortex immunofluorescence density in Iba-1, n = 4. (E, F, G, H) Western blot analysis of Iba-1, cleaved Gasdermin D and NLRP3 in visual cortex lysates. The molecular mass is indicated in kilodaltons, n = 3. (I, J) Quantitative analysis of representative fluorescence images of NeuN staining with TUNEL labeling in the peri-contusion region from different groups that information is shown in images, Scale bar = 100 μm, n = 4. (K) Quantitative analysis of visual cortex NeuN+ cells. (L) Representative images of immunofluorescence staining for MBP (green) and βIII-Tubulin (red) in mouse optic nerve slices. Group information is shown in images. Scale bar = 20 μm, n = 4. (M) optic nerve MBP density in different groups, n = 4. (N) optic nerve relative fluorescence intensity of MBP + /β III Tubulin + in different groups, n = 4. (O) TEM analysis of the optic nerve between the BE 24 h and BE 28 d groups, red stars indicate axons with obvious demyelination. Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, ns p > 0.05.

Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with gentle agitation using the following primary antibodies: anti-MBP (1:10,000; CST) anti-Iba-1 (1:10,000; CST), anti-NLRP3 (1:10,000; Invitrogen), anti-IL-1β (1:8,000; CST, # 12242) anti-cleaved Gasdermin D (1:10,000, CST, # 10173), and anti-GAPDH (1:10,000, Abcam, # ab9485).

Techniques: Immunofluorescence, Staining, Fluorescence, Western Blot, TUNEL Assay, Labeling